糖心原创

Antibacterial Potential of Locally Formulated Disinfectant/Antiseptic from Nipa Bioethanol

James Paul T. Madigal | Thiara Celine E. Suarez | Karyl Mae D. Ramos | Jayson F. Cariaga | Shirley C. Agrupis

 

Abstract:

Ethyl Alcohol, 70% was formulated from Nipa Bioethanol, “Nipahol”, to augment the dwindling supply of alcohol to fight COVID-19. Nipahol is high-grade bioethanol produced from nipa sap using the proprietary fermentation and distillation technologies developed by a group of researchers at the MMSU-NBERIC. For more than a decade now, MMSU has been implementing RDE for the production of fuel-grade ethanol from non-traditional sugar feedstocks like nipa sap in support of the Biofuel Act of 2006. To ensure the quality and efficacy of the formulated product, the present study was set to explore the antibacterial potential of the locally formulated nipa disinfectant/antiseptic as an alternative alcohol formulation amid the COVID-19 pandemic using standard microbiological assays. Susceptibility test revealed that 70% and 80% nipa alcohol formulations showed inhibitory activity against Staphylococcus aureus (6.25 mm and 4.25 mm zone of inhibitions). The 95% nipa alcohol concentration showed a bactericidal effect against Escherichia coli and S. aureus. High percent (%) bacterial cell reduction (90-99.9% log re-duction) was observed when alcohol concentration and time increases. A confirmatory antimicrobial susceptibility test conducted by DOST-RO1 Microbiology Division reported that 95% nipa alcohol showed an active inhibitory effect to test organisms while partial active observed in 70% nipa alcohol formulation. Glo-Germ Test revealed nipa disinfectant/antiseptic is as effective as commercial alcohol, thus, it can be utilized as an alternative intervention to prevent the spread of infectious microorganisms. The effectiveness of nipa disinfectant/antiseptic formulations is heightened with proper handwashing, strictly following proper hygiene, and health protocols. In conclusion, the formulated nipa disinfectant/antiseptic possesses the antibacterial potential to inhibit the multiplication of E. coli and S. aureus.