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Home⇛The Philippine Journal of Biochemistry and Molecular Biology (PJBMB)⇛vol. 1 no. 1 & 2 (2020)

Preliminary Evaluation of rbcL, matK, and SRAP Markers for the Molecular Characterization of Five Philippine Allium sativum varieties

Allisandra Isabel B. Bigtas | Patrick Gabriel G. Moreno | Francisco M. Heralde Iii

Discipline: agriculture, food and related studies

 

Abstract:

Alliumsativum, commonly known as “bawang”, is one of the economically important crops in the Philippines mainly for spice and other uses including herbalmedicine for hypertension and cancer. Several studies showing therapeutic properties of garlic often do not disclose the variety used while typical varietal identification rely on phenotypic or agronomic traits which may be inadequate to define distinct variants. This study aimed to evaluate the utility of chloroplast genes, rbcL andmatK, aswell asSequence-RelatedAmplifiedPolymorphism(SRAP) to define and characterize fiveA. sativumvarieties grown in the Philippines. Five garlic varieties (i.e., GNT4, GMX6, IP3A, GMR10 and GTB17) were collected from a demo farm in Batac, Ilocos Norte, Philippines. The bulbs were made to grow roots which were subsequently used for DNA extraction and PCR analysis. Results showed that PCR amplified sequences of rbcL and matK genes were able to identify A. sativum varieties with ample discriminatory power to differentiate closely related species. The identified SRAP markers were also able to showa degree of polymorphismamong the five local varieties. The rbcL sequenceswere highly conserved, the matK-sequences were able to discriminate GTB17 while the SRAP using the primer combinations ME1-EM3 and ME3- EM3 discriminated all the five varieties.Asummary of the concatenatedmolecular features of the five varietieswas provided. Albeit preliminary, the studywas able to showthe potential ofmatK and SRAP in discriminating the local A. sativumvarieties from each other and from other varieties of different origin.



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