糖心原创

Home⇛Recoletos Multidisciplinary Research Journal⇛vol. 1 no. Special Issue (2025)

The Antiangiogenic Activity of the Pereskia grandifolia (Seven Star Needle) Crude Leaf Extract Using the Chick Embryo Chorioallantoic Membrane (CAM) Assay

Aline Abala | Anna Teresa Acu帽a | Lojsam Daan | Jonessa Pearl Dico | Aleana Dannyze Fontillas | Kevin Christian Dela Cruz

Discipline: biomedical sciences (non-specific)

 

Abstract:

Background: The study focused on the anticancer potential of Pereskia grandifolia, specifically its antiangiogenic properties. Methods: Soxhlet extraction and rotary evaporation yielded a crude leaf extract containing flavonoids, phenols, tannins, steroids, and alkaloids. An ex-ovo Chorioallantoic Membrane (CAM) Assay was performed on twentyone 4-day-old chicken embryos, divided into three groups: the experimental group (40.5 mg/50 mcL crude extract), positive control (7.5 mcg/50 mcL dexamethasone), and negative control (50 mcL distilled water). Results: The experimental group exhibited the highest inhibition of blood vessel length (43.99%), significantly greater than the positive control group (28.51%) and negative control group (0%). Statistical analysis confirmed significant differences in blood vessel length inhibition (t = 2.590, p = 0.024), number (F = 93.415, p < 0.001, w2 = 0.80), and vessel density (F = 94.343, p < 0.001). Conclusion: These findings suggest Pereskia grandifolia's potential as an antiangiogenic agent for cancer therapy.



References:

  1. Bactin, J. M. A., Diapana, K. T. P., Inocentes, B. M. J., Mercado, J. A. M., & Simbajon, J. A. R. (2022). The antiangiogenic activity of carotenoid from Daucus carota (carrot) peels using the chick embryo chorioallantoic membrane (CAM) model [Unpublished undergraduate thesis]. Cebu Doctors’ University.
  2. Bhandari, K., Singla, R. K., De, B., Ghosh, B. C., Katakam, P., Khushwaha, D. K., Gundamaraju, R., Sen, G., Saha, G., & Mitra, A. (2015). Chemometrics based extraction of polyphenolics from fresh tea leaves and processed tea showing in- silico docking and antioxidative theronostic dietary adjuvant in alzheimer. Indo Global Journal of Pharmaceutical Sciences, 5(3). 
  3. De Souza, A. H., Mendonca, H. O., Paula, A. C., Augusti, R., Fante, C. A., Melo, J. O., & Carlos, L. A., (2022). Influence of harvest time on the chemical profile of Pereskia aculeate Mill. using paper spray mass spectrometry. Molecules, 27(13), 4276. 
  4. Dohle, D. S., Pasa, S. D., Gustmann, S., Laub, M., Wissler, J. H., Jennissen, H. P., & Dünker, N. (2009). Chick ex ovo culture and ex ovo CAM assay: How it really works. Journal of Visualized Experiments, 33, 1620. 
  5. Hassan, L. E., Ahamed, M. K., Majid, A. A., Baharetha, H. M., Muslim, N. S., Nassar, Z. D., & Majid, A.M.S.A. (2014). Correlation of antiangiogenic, antioxidant and cytotoxic activities of some Sudanese medicinal plants with phenolic and flavonoid contents. BMC Complementary and Alternative Medicine, 14(406). 
  6. Johari, M. A., & Khong, H. Y. (2019). Total phenolic content and antioxidant and antibacterial activities of Pereskia bleo. Advances in Pharmacological Sciences. 
  7. Kancherla, N., Dhakshinamoothi, A., Chitra, K., & Komaram, R. B. (2019). Preliminary analysis of phytoconstituents and evaluation of anthelminthic property of Cayratia auriculata (in vitro). Maedica: A Journal of Clinical Medicine, 14(4), 350-356. 
  8. Kintek Solution Ltd. (n.d.). What temperature should methanol be in rotary evaporator? 
  9. Liu, B., & Goodwin, J. E. (2020). The effect of glucocorticoids on angiogenesis in the treatment of solid tumors. Journal of Cellular Signaling, 1(3), 42-49. 
  10. Liu, J., Zhang, X., Li, G., Li, Xu, F., Li, S., Teng, L., Li, Y., & Sun, F. (2019). Anti-angiogenic activity of bevacizumab-bearing dexamethasone-loaded PLGA nanoparticles for potential intravitreal applications. International Journal of Nanomedicine, 14, 8819-8834. 
  11. López-Camarillo, C., Ruiz-Garcia, E., Starling, N., & Marchat, L. A. (2020). Neovascularization, angiogenesis and vasculogenic mimicry in cancer. Frontiers in Oncology, 10. 
  12. Maciel, V. B. V., Yoshida, C. M. P., & Goycoolea, F. M. (2019). Agronomic cultivation, chemical composition, functional activities and applications of Pereskia species – A mini review. Current Medicinal Chemistry, 26(24), 4573-4584. 
  13. Manaf, S. F. A., Aziz, N. S. A., Abdullah, S., Raslan, R., Hamzah, F., & Alias, N. H. (2014). Optimization of drying and extraction process of Pereskia bleo leaves in determining antioxidant properties utilizing Pareto ANOVA. Applied Mechanics and Materials, 548–549, 96–100. 
  14. Mohan, C. (2006). Buffers: A guide for the preparation and use of buffers in biological systems (3rd ed.). 
  15. National Cancer Institue. (2018, April 2). Angiogenesis inhibitors. 
  16. National University of Singapore. (2019, May 12). Anticancer properties uncovered in plants. NUS News. 
  17. Ngelangel, C. A., Lam, H. Y., Rivera, A. S., Kimman, M. L., Real, I. O., & Balete, S. L. (2018). Philippine costs in oncology (PESO): Describing the economic impact of cancer on Filipino cancer patients using the ASEAN costs in oncology study dataset. Acta Medica Philippina, 52(2). 
  18. Nichols-Orians, C. (1992). The acceptability of young and mature leaves to leaf-cutter ants varies with light environment. Biotropica, 24(2), 211–214. 
  19. Pinto, M. T., Ribeiro, A. S., Conde, I., Carvalho, R., & Paredes, J. (2020). The chick chorioallantoic membrane model: A new in vivo tool to evaluate breast cancer stem cell activity. International Journal of Molecular Sciences, 22(1), 334. 
  20. Rohrig, B. (2015, December). Safety data sheets: Information that could save your life. American Chemical Society. 
  21. Siska, S., Hanani, H., Bariroh, T., Febrianto, B., Pratiwi, A. D., Yaner, N. N., & Fitri, N. A. (2023). Effect of the ethanol extract of Pereskia bleo (Kunth) DC. on the blood pressure and electrolyte levels of hypertensive rats. Journal of Herbmed Pharmacology, 12(3), 448-452. 
  22. Smith, G. L., Lopez-Olivo, M. A., Advani, P. G., Ning, M. S., Geng, Y., Giordano, S. H., & Volk, R. J. (2019). Financial burdens of cancer treatment: A systematic review of risk factors and outcomes. Journal of the National Comprehensive Cancer Network, 17(10), 1184-1192.
  23. Tan, M.L., Sulaiman, S. F., Najimuddin, N., Samian, M. R., & Tengku Muhammad, T. S. (2005). Methanolic extract of Pereskia bleo (Kunth) DC. (Cactaceae) induces apoptosis in breast carcinoma, T47-D cell line. Journal of Ethnopharmacology, 96(1-2), 287–294. . 2004.09.025
  24. Umar, M. I., Asmawi, M. Z., Sadikun, A., Majid, A. M. S. A., Al-Suede, F. S. R., Hassan, L. E. A., Altaf, R., & Ahamed, M. B. K. (2014). Ethyl-p-methoxycinnamate isolated from Kaempferia galanga inhibits inflammation by suppressing interleukin-1, tumor necrosis factor-α, and angiogenesis by blocking endothelial functions. Clinics, 69(2), 134–144. 
  25. University of Wisconsin. (2019, March). Leaf cactus, Pereskia spp.- Master gardener program. . wisc.edu/files/2019/03/Pereskia.pdf
  26. Uslu, S., Emon, S. T., Alaca, N., Kocaturk, D. C., Uslu, U., & Uysal, A. (2022). Effects of dexamethasone exposure on neural crest cells and primary and secondary neurulation in chick embryos. Turkish Neurosurgery, 32(3), 412–419. 
  27. World Health Organization [WHO]. (2022, February 3). Cancer. 
  28. Zareisedehizadeh, S., Tan, C. K., & Koh, H. L. (2014). A review of botanical characteristics, traditional usage, chemical components, pharmacological activities, and safety of Pereskia bleo (Kunth) DC. Evidence-based Complementary and Alternative Medicine. 

Tools


Copyright © 2026  糖心原创 Exclusively distributed by